Soybean is an important oil crop in China. In the process of determining the oil content of fighting, it is mainly divided into two methods: instrumentation and chemical determination. For the determination of soybean seed fat, attention should be paid to the influence of temperature and time, and the measured instrument will also have an effect. The fat analyzer is a measuring device for fat content, and its measurement result is more accurate and reliable.

We found in the fat analyzer test that not only the water bath temperature and the time of reflux of the extractant (ether) had an effect on the measurement results, but also the ambient temperature (room temperature) of the extractor directly affected the degree of fat extraction. . For this reason, we have also done the effect of different room temperature on the determination of fat content in Heinong 26. At a water temperature of 80°C, it was extracted with 500 ml and refluxed for 8, 10, 12, and 14 hours. Four treatments were performed at room temperature 15-20°C, and the results were all higher than those at room temperature 7-8°C. To test the reliability of the difference, a t-test was conducted. The data obtained by the YG-2 method in the same hole water bath temperature (8o°C), 4 different extraction times, and two different room temperature conditions (15 to 20°C and 7 to 8°C), the t test was significant at 3 points, and the other point was not. Significant. The 50Oml extraction method, at the same water temperature, was not significant at 4 o'clock. This shows that the room temperature has an effect on the YG-2 method and has little effect on the 500ml extraction method, and has almost no effect on the oil weight method.

50oml extraction, reflux at room temperature 15-20°C. The hourly extraction of fat was the highest, but its value was not significant compared with 8 hours. Similarly, the same 8 hours and 12 and 14 hours compared to one is not significant, so the room temperature 15-20 ° C reflux 8 hours, the water temperature of 80 ° C for 50Oml extraction of the best conditions. Type YG-2 at room temperature 15-20 °C, water temperature 80 °C, 4 extraction time, the measured results have a slowly rising trend, with 8 hours as the highest, but compared with the average of 5 repetitions in 6 hours, The absolute difference was only 0.02%, and the difference in t-tests was not significant. Therefore, we believe that the extraction time can be saved at room temperature 15-20%. It can be used as the best extraction time of YG-2 method when it is refluxed on a water bath at 80°C for 6 hours.

In summary, the fat analyzer can determine the fat content of oil crop seeds with a small amount of sample. Generally, the oil weight method (arbitration method) can be used. At a room temperature of 15 to 20°C, the water bath temperature is 75°C and the extraction time is 8 hours. If a large number of samples are measured, in order to improve the work efficiency, a residual method-YG-2 type extraction method may be adopted. At a room temperature of 15-20°C, the water bath temperature is 80°C and extraction is performed for 6-8 hours. 8 to 10 hours can be extracted for samples with high oil content.

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