In the current process of vigorously promoting the revitalization of the seed industry, the protection of germplasm resources has become the focus of the industry. In order to effectively protect these good genetic genes in nature, we need to understand the preservation methods of germplasm resources? Generally speaking, the preservation methods of germplasm resources mainly include dry and sealed preservation, cryopreservation, off-site preservation, germplasm resource bank preservation, nature reserve preservation, germplasm resource nursery preservation, test tube seedling preservation, ultra-low temperature preservation, and ultra-dry seeds. There are many kinds of storage, but at present, the construction of a germplasm resource bank is currently a more popular and reliable technical solution for low-temperature and low-humidity preservation. 1. Dry and airtight storage : a method of storing seeds in a sealed container with an appropriate amount of desiccant at room temperature. The desiccant can be quicklime, calcium chloride, silica gel, etc. Generally, the ratio of seed to desiccant is 1:2, and the ratio of silica gel can be appropriately increased. 2. Low-temperature storage: After the seeds are dried, they are sealed and packaged, and then stored under low-temperature conditions such as cold storage or refrigerator. This is a method where low temperature does not control humidity. Therefore, special attention should be paid to the container for storing seeds to be strictly sealed to prevent moisture absorption. 3. Storage in different places: move the seeds from humid areas to dry, low-temperature areas for storage. The climate in Qinghai, Xinjiang and other places in my country is dry and cold. Xining, Qinghai has an annual average temperature of 6.0℃ and an average relative humidity of 53.9%. The relative humidity from July to September is around 65%, which is equivalent to a natural low-temperature dry warehouse. The common feature of the above preservation methods is that the seed water content is still relatively high. Metabolism of seeds is still relatively vigorous in storage, and seeds age quickly. The temperature of seeds often changes with the changes of the external environment. If the sealing is not good, the moisture content of the seeds will be balanced with the environmental humidity. 4. Germplasm resource bank preservation: there are humidity control equipment in the germplasm resource bank , which is an ideal condition for the preservation of germplasm resources today. According to the storage period, it can be divided into short-term storage, medium-term storage and long-term storage. Short-term storage: the storage temperature is controlled at 10℃~15℃, and the relative humidity is 50%~60%. The seeds are packaged in paper bags or cloth bags, and generally can be stored for about 5 years as temporary application materials for identification, research and distribution. Mid-term storage: storage temperature 0℃~10℃, relative humidity below 50%. The seeds are packaged and sealed with moisture-proof material. Seeds have a moisture content of about 8% and can be stored for more than 15%. Preserved seeds are mainly used as distribution materials. Long-term storage: storage temperature is -10℃~-20℃, relative humidity is 30%~50%, and seed moisture content is 4%~6%. The seeds are sealed in aluminum boxes or aluminum foil plastic packaging, or vacuum sealed. The storage period is 50 to 100 years. Long-term storage is generally not used for distribution, that is, when the distributed materials are used up, they can be used for the extraction of propagation materials, so it is also called the basic library. Duplicate storage in different places: Because all the seed resources are stored in one place, it is very dangerous. In order to prevent accidents, a part of the seed is separated and placed in another place for duplicate storage. This kind of preservation method is called duplicate storage in another place. 5. Preservation in nature reserves: It is the best way to preserve certain wild germplasm resources, and it is also preserved in situ. Keep the original ecological environment so that they will not become extinct with the disappearance of their natural habitats. 6. Preservation of germplasm resources in the nursery: For perennial plants such as trees, tea trees, aquatic vegetables, etc., live plants are preserved in the field and germplasm is preserved by asexual reproduction. 7. Test-tube seedling preservation: It is difficult to store asexually propagated crops with seeds, and it can be preserved in test-tube seedlings, also known as meristem preservation. The method is to remove the plant stems and inoculate them in a test tube with a differentiation medium, and then take roots and grow stems under suitable temperature and light. Then transfer it to a test tube containing a growth medium. When the seedling grows to about 10 cm, it can be stored at low temperature. Generally, after half a year to one year, remove the test tube stems and put them in a new test tube. Continue to store as described above, and transfer the tube once every six months, so that it can be stored indefinitely. 8. Ultra-low temperature storage: preserve germplasm resources below -80°C. Ultra-low temperature preservation of germplasm resources generally uses liquid nitrogen as a cold source, and the temperature in liquid nitrogen is -196°C. Under such low temperature, the metabolic processes of protoplasm, cells, tissues, organs or seeds are basically stopped and are in a state of "vital suspension", which greatly reduces or stops the deterioration related to metabolism, thus creating conditions for "indefinite" preservation. 9. Storage of ultra-dry seeds: For those seeds that can reach ultra-dry (less than 5% to 7% of the seed moisture content) and are harmless, use the ultra-dry sealed package and store at room temperature to save energy. WenZhou Hongtu Automobile Parts Co.,Ltd , https://www.hongtuautomobileparts.com